Project A1

Cross-talk of Aspergillus fumigatus with neutrophilic granulocytes

Neutrophils are the most important effector cells in the immune response against Aspergillus fumigatus. In project A1, we aim at understanding the mechanisms how neutrophils control A. fumigatus infections.

In the first funding period we developed novel methods for the bioinformatics-based analysis of live cell imaging. This allowed automated quantification of phagocytosis and the identification of fungal molecules inhibiting phagocytosis. Furthermore, quantitative analysis of the dual proteome of A. fumigatus and neutrophils by LC-MS was established. This led to the discovery of novel human proteins involved in the interactions with this fungus.

In the second funding period our research will focus on the identification of novel effector mechanisms of neutrophils against A. fumigatus, building on our discoveries from the first funding period. We will also characterize immune cell receptors potentially involved in the cross-talk of neutrophils with A. fumigatus. For this, we will use immune cells from knockout mice, cell lines, monoclonal antibodies that block specific receptors and mouse infection models for invasive aspergillosis. Finally, we will analyze patient groups for single nucleotide polymorphisms (SNPs) in the proteins that interact with A. fumigatus as basis for translational approaches.

Summary of research to characterize the interaction of different A. fumigatus morphotypes with neutrophilic granulocytes
Summary of research to characterize the interaction of different A. fumigatus morphotypes with neutrophilic granulocytes
Principal Investigator
Publications
Author Year Title Journal Links
Goldmann M, Schmidt F, Cseresnyés Z, Orasch T, Jahreis S, Hartung S, Figge MT, von Lilienfeld-Toal M, Heinekamp T, Brakhage AA 2023 The lipid raft-associated protein stomatin is required for accumulation of dectin-1 in the phagosomal membrane and for full activity of macrophages against Aspergillus fumigatus. mSphere 8(1) :e0052322 PubMed
Orasch T, Gangapurwala G, Vollrath A, González K, Alex J, De San Luis A, Weber C, Hoeppener S, Cseresnyés Z, Figge MT, Guerrero-Sanchez C, Schubert US, Brakhage AA 2023 Polymer-based particles against pathogenic fungi: A non-uptake delivery of compounds. Biomater Adv 146, 213300 PubMed
Jia LJ, Rafiq M, Radosa L, Hortschansky P, Cunha C, Cseresnyés Z, Krüger T, Schmidt F, Heinekamp T, Straßburger M, Löffler B, Doenst T, Lacerda JF, Campos A, Figge MT, Carvalho A, Kniemeyer O, Brakhage AA 2023 Aspergillus fumigatus hijacks human p11 to redirect fungal-containing phagosomes to non-degradative pathway. Cell Host & Microbe: 31,3 Cell Host & Microbe
Kelani AA, Bruch A, Rivieccio F, Visser C, Krueger T, Weaver D, Pan X, Schaeuble S, Panagiotou G, Kniemeyer O, Bromley MJ, Bowyer P, Barber AE, Brakhage AA, Blango MG. 2023 Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptome. RNA  [Epub ahead of print] PubMed
Sala A, Ardizzoni A, Spaggiari L, Vaidya N, van der Schaaf J, Rizzato C, Cermelli C, Mogavero S, Krüger T, Himmel M, Kniemeyer O, Brakhage AA, King BL, Lupetti A, Comar M, de Seta F, Tavanti A, Blasi E, Wheeler RT, Pericolini E. A 2023 New Phenotype in Candida-Epithelial Cell Interaction Distinguishes Colonization- versus Vulvovaginal Candidiasis-Associated Strains. mBio 3: e0010723 PubMed
Brioli A, Gengenbach L, Mancuso K, Binder M, Ernst T, Heidel FH, Stauch T, Zamagni E, Hilgendorf I, Hochhaus A, Engelhardt M, von Lilienfeld-Toal M. 2023 Pomalidomide combinations are a safe and effective option after daratumumab failure. J Cancer Res Clin Oncol. [2]13 PubMed
Giesen N, Busch E, Schalk E, Beutel G, Rüthrich MM, Hentrich M, Hertenstein B, Hirsch HH, Karthaus M, Khodamoradi Y, Koehler P, Krüger W, Koldehoff M, Krause R, Mellinghoff SC, Penack O, Sandherr M, Seggewiss-Bernhardt R, Spiekermann K, Sprute R, Stemler J, Weissinger F, Wörmann B, Wolf HH, Cornely OA, Rieger CT, von Lilienfeld-Toal M 2023 AGIHO guideline on evidence-based management of COVID-19 in cancer patients: 2022 update on vaccination, pharmacological prophylaxis and therapy in light of the omicron variants. Eur J Cancer. 181: 102-118 PubMed
Krespach MKC, Stroe MC, Netzker T, Rosin M, Zehner LM, Komor AJ, Beilmann JM, Krüger T, Scherlach K, Kniemeyer O, Schroeckh V, Hertweck C, Brakhage AA 2023 Streptomyces polyketides mediate bacteria–fungi interactions across soil environments. Nature Microbiology Nat Microb
Garloff V, Krüger T, Brakhage A, Rubio I. 2023 Control of TurboID-dependent biotinylation intensity in proximity ligation screens. J Proteomics. J Proteomics 15;279:104886 PubMed
Mirhakkak MH, Chen X, Ni Y, Heinekamp T, Sae-Ong T, Xu LL, Kurzai O, Barber AE, Brakhage AA, Boutin S, Schäuble S, Panagiotou G 2023 Genome-scale metabolic modeling of Aspergillus fumigatus strains reveals growth dependencies on the lung microbiome. Nat Commun. 14(1):4369 PubMed
Wein P, Dornblut K, Herkersdorf S, Krüger T, Molloy EM, Brakhage AA, Hoffmeister D, Hertweck C 2023 Bacterial secretion systems contribute to rapid tissue decay in button mushroom soft rot disease. mBio: e0078723 PubMed
Garloff V, Krüger T, Brakhage A, Rubio I. 2023 Control of TurboID-dependent biotinylation intensity in proximity ligation screens. J Proteomics 279:104886 PubMed
Sala A, Ardizzoni A, Spaggiari L, Vaidya N, van der Schaaf J, Rizzato C, Cermelli C, Mogavero S, Krüger T, Himmel M, Kniemeyer O, Brakhage AA, King BL, Lupetti A, Comar M, de Seta F, Tavanti A, Blasi E, Wheeler RT, Pericolini E   2023 A new phenotype in Candida-epithelial cell interaction distinguishes colonization-versus culvovaginal candidiasis-associated strains. mBio 14(2), e0010723 PubMed
Ramírez-Zavala B, Betsova D, Schwanfelder S, Krüger I, Mottola A, Krüger T, Kniemeyer O, Brakhage AA, Morschhäuser J. 2023 Multiple phosphorylation sites regulate the activity of the repressor Mig1 in Candida albicans. mSphere: 8 (6) PubMed
Schruefer S, Pschibul A, Wong SSW, Sae-Ong T, Wolf T, Schäuble S, Panagiotou G, Brakhage AA, Aimanianda V, Kniemeyer O, Ebel F 2023 Distinct transcriptional responses to fludioxonil in Aspergillus fumigatus and its ΔtcsC and Δskn7 mutants reveal a crucial role for Skn7 in the cell wall reorganizations triggered by this antifungal. BMC Genomics. 14;24(1):684 PubMed
Halder LD, Babych S, Palme DI, Mansouri-Ghahnavieh E, Ivanov L, Ashonibare V, Langenhorst D, Prusty B, Rambach G, Wich M, Trinks N, Blango MG, Kornitzer D, Terpitz U, Speth C, Jungnickel B, Beyersdorf N, Zipfel PF, Brakhage AA, Skerka C 2022 Candida albicans induces cross-kingdom miRNA trafficking in human monocytes to promote fungal growth. mBio 13(1): e0356321 mBio
Rafiq M, Rivieccio F, Zimmermann AK, Visser C, Bruch A, Krüger T, González Rojas K, Kniemeyer O, Blango MG, Brakhage AA 2022 PLB-985 neutrophil-like cells as a model to study Aspergillus fumigatus pathogenesis. mSphere e0094021 PubMed
Hoang TNM, Cseresnyés Z, Hartung S, Blickensdorf M, Saffer C, Rennert K, Mosig AS, von Lilienfeld-Toal M, Figge MT 2022 Invasive aspergillosis-on-chip: A quantitative treatment study of human Aspergillus fumigatus infection Biomaterials 283: 121420 PubMed
Brandt P, Gerwien F, Wagner L, Krüger T, Ramirez-Zavala B, Mirhakkak MH, Schäuble S, Kniemeyer O, Panagiotou G, Brakhage AA, Morschhäuser J, Vylkova S 2022 Candida albicans SR-Like Protein Kinases Regulate Different Cellular Processes: Sky1 Is Involved in Control of Ion Homeostasis, While Sky2 Is Important for Dipeptide Utilization. Frontiers Cellular Infection Microbiology doi:0.3389/fcimb.2022.850531 Front. Cell. Infect. Microbiol.
Fendler A, de Vries EGE, GeurtsvanKessel CH, Haanen JB, Wörmann B, Turajlic S, von Lilienfeld-Toal M 2022 COVID-19 vaccines in patients with cancer: immunogenicity, efficacy and safety. Nat Rev Clin Oncol , 1-17. PubMed
Goldman JD, Gonzalez MA, Rüthrich MM, Sharon E, von Lilienfeld-Toal M. 2022 COVID-19 and Cancer: Special Considerations for Patients Receiving Immunotherapy and Immunosuppressive Cancer Therapies. Am Soc Clin Oncol Educ Book  doi: 10.1200/EDBK 359656. PubMed
Niemiec MJ, Kapitan M, Himmel M, Döll K, Krüger T, Köllner TG, Auge I, Kage F, Alteri CJ, Mobley HLT, Monsen T, Linde S, Nietzsche S, Kniemeyer O, Brakhage AA, Jacobsen ID 2022 Augmented Enterocyte Damage During Candida albicans and Proteus mirabilis Coinfection. Front Cell Infect Microbiol. 12:866416 PubMed
Balkenhol J,  Bencurova E, Gupta SK, Schmidt H, Heinekamp T, Brakhage AA,  Pottikkadavath A, Dandekar T 2022 Prediction and validation of host-pathogen interactions by a versatile inference approach using Aspergillus fumigatus as a case study. Computational and Structural Biotechnology Journal 20: 4225-4237 PubMed
Tappe B, Lauruschkat CD, Strobel L, Pantaleón García J, Kurzai O, Rebhan S, Kraus S, Pfeuffer-Jovic E, Bussemer L, Possler L, Held M, Hünniger K, Kniemeyer O, Schäuble S, Brakhage AA, Panagiotou G, White PL, Einsele H, Löffler J, Wurster S. 2022 COVID-19 patients share common, corticosteroid-independent features of impaired host immunity to pathogenic molds. Front Immunol. 13: 954985 PubMed
Böttcher B, Driesch D, Krüger T, Garbe E, Gerwien F, Kniemeyer O, Brakhage AA, Vylkova S 2022 Impaired amino acid uptake leads to global metabolic imbalance of Candida albicans biofilms. NPJ Biofilms Microbiomes. 8(1): 78 PubMed
Montaño DE, Hartung S, Wich M, Ali R, Jungnickel B, von Lilienfeld-Toal M, Voigt K 2022 The TLR-NF-kB axis contributes to the monocytic inflammatory response against a virulent strain of Lichtheimia corymbifera, a causative agent of invasive mucormycosis. Front Immunol. 13: 882921 PubMed
de Assis LJ, Silva LP, Bayram O, Dowling P, Kniemeyer O, Krüger T, Brakhage AA, Chen Y, Dong L, Tan K, Wong KH, Ries LNA, Goldman GH 2021 Carbon catabolite repression in filamentous fungi is regulated by phosphorylation of the transcription factor CreA. mBio 12: e03146-20 PubMed
Yu Y, Wolf AK, Thusek S, Heinekamp T, Bromley M, Krappmann S, Terpitz U, Voigt K, Brakhage AA, Beilhack A 2021 Direct visualization of fungal burden in filamentous fungus-infected silkworms. J Fungi (Basel) 7: 136 PubMed
Strømland Ø, Kallio JP, Pschibul A, Skoge RH, Harðardóttir HM, Sverkeli LJ, Heinekamp T, Kniemeyer O, Migaud M, Makarov MV, Gossmann TI, Brakhage AA, Ziegler M 2021 Discovery of fungal surface NADases predominantly present in pathogenic species. Nat Commun 12: 1631 PubMed
Lauruschkat CD, Page L, White PL, Etter S, Davies HE, Duckers J, Ebel F, Schnack E, Backx M, Dragan M, Schlegel N, Kniemeyer O, Brakhage AA, Einsele H, Loeffler J, Wurster S 2021 Development of a simple and robust whole blood assay with dual co-stimulation to quantify the release of T-cellular signature cytokines in response to Aspergillus fumigatus antigens. J Fungi 7: 462 MDPI
Page L, Wallstabe J, Lother J, Bauser M, Kniemeyer O, Strobel L, Voltersen V, Teutschbein J, Hortschansky P, Morton CO, Brakhage AA, Topp M, Einsele H, Wurster S, Loeffler J 2021 CcpA- and Shm2-pulsed myeloid dendritic cells induce T-cell activation and enhance the neutrophilic oxidative burst response to Aspergillus fumigatus. Front Immunol 12: 659752 PubMed
Brakhage AA, Zimmermann AK, Rivieccio F, Visser C, Blango MG 2021 Host-derived extracellular vesicles for antimicrobial defense. microLife 2: uqab003 microLife
Gupta SK, Srivastava M, Osmanoglu Ö, Xu Z, Brakhage AA, Dandekar T 2021 Aspergillus fumigatus versus Genus Aspergillus: Conservation, adaptive evolution, and specific virulence genes. Microorganisms 9: 2014 PubMed
Mogavero S, Sauer FM, Brunke S, Allert S, Schulz D, Wisgott S, Jablonowski N, Elshafee O, Krüger T, Kniemeyer O, Brakhage AA, Naglik JR, Dolk E, Hube B 2021 Candidalysin delivery to the invasion pocket is critical for host epithelial damage induced by Candida albicans. Cell microbiol 23: e13378 PubMed
Ewald J, Rivieccio F, Radosa L, Schuster S, Brakhage AA, Kaleta C 2021 Dynamic optimization reveals alveolar epithelial cells as key mediators of host defense in invasive aspergillosis. PLoS Comp Bio 17: e1009645 PubMed
Wich M, Greim S, Ferreira-Gomes M, Krüger T, Kniemeyer O, Brakhage AA, Jacobsen ID, Hube B, Jungnickel B 2021 Functionality of the human antibody response to Candida albicans. Virulence 12: 3137-48 PubMed
López-Berges MS, Scheven MT, Hortschansky P, Misslinger M, Baldin C, Gsaller F, Werner ER, Krüger T, Kniemeyer O, Weber J, Brakhage AA, Haas H 2021 The bZIP Transcription Factor HapX Is Post-Translationally Regulated to Control Iron Homeostasis in Aspergillus fumigatus. Int J Mol Sci. 22(14): 7739 PubMed
Radosa S, Sprague JL, Lau SH, Tóth R, Linde J, Krüger T, Sprenger M, Kasper L, Westermann M, Kniemeyer O, Hube B, Brakhage AA, Gácser A, Hillmann F 2021 The fungivorous amoeba Protostelium aurantium targets redox homeostasis and cell wall integrity during intracellular killing of Candida parapsilosis. Cell Microbiol. 23(11): e13389 PubMed
Shopova IA, Belyaev I, Dasari P, Jahreis S, Stroe MC, Cseresnyés Z, Zimmermann AK, Medyukhina A, Svensson CM, Krüger T, Szeifert V, Nietzsche S, Conrad T, Blango MG, Kniemeyer O, Lilienfeld-Toal Mv, Zipfel PF, Ligeti E, Figge MT, Brakhage AA 2020 Human neutrophils produce antifungal extracellular vesicles against Aspergillus fumigatus. mBio 11: e00596-20 PubMed
Blango MG, Pschibul A, Rivieccio F, Krüger T, Muhammad R, Jia L, Zheng T, Goldmann M, Voltersen V, Li Jun, Panagiotou G, Kniemeyer O, Brakhage AA 2020 Dynamic surface proteomes of allergenic fungal conidia. J Proteome Res 19: 2092-104 PubMed
Hassan MIA, Kruse J, Krüger T, Dahse HM, Cseresnyés Z, Blango MG, Slevogt H, Hörhold F, Ast V, König R, Figge MT, Kniemeyer O, Brakhage AA, Voigt K 2020 Functional surface proteomic profiling reveals the host heat-shock protein A8 as a mediator of Lichtheimia corymbifera recognition by murine alveolar macrophages. Environ Microbiol 22: 3722-40 PubMed
Ruben S, Garbe E, Mogavero S, Albrecht-Eckardt D, Hellwig D, Häder A, Krüger T, Gerth K, Jacobsen ID, Elshafee O, Brunke S, Hünniger K, Kniemeyer O, Brakhage AA, Morschhäuser J, Hube B, Vylkova S, Kurzai O, Martin R 2020 Ahr1 and Tup1 contribute to the transcriptional control of virulence-associated genes in Candida albicans. mBio 11: e00206-20 PubMed
Gonçalves SM, Duarte-Oliveira C, Campos CF, Aimanianda V, Ter Horst R, Leite L, Mercier T, Pereira P, Fernández-García M, Antunes D, Rodrigues CS, Barbosa-Matos C, Gaifem J, Mesquita I, Marques A, Osório NS, Torrado E, Rodrigues F, Costa S, Joosten LA, Lagrou K, Maertens J, Lacerda JF, Campos A Jr, Brown GD, Brakhage AA, Barbas C, Silvestre R, van de Veerdonk FL, Chamilos G, Netea MG, Latgé JP, Cunha C, Carvalho A 2020 Phagosomal removal of fungal melanin reprograms macrophage metabolism to promote antifungal immunity. Nat Commun 11: 2282 PubMed
Jia LJ, Krüger T, Blango MG, von Eggeling F, Kniemeyer O, Brakhage AA 2020 Biotinylated surfome profiling identifies potential biomarkers for diagnosis and therapy of Aspergillus fumigatus infection. mSphere 5: e00535-20 PubMed
Schmidt F, Thywißen A, Goldmann M, Cunha C, Cseresnyés Z, Schmidt H, Rafiq M, Galiani S, Gräler MH, Chamilos G, Lacerda JF, Campos Jr A, Eggeling C, Figge MT, Heinekamp T, Filler SG, Carvalho A, Brakhage AA 2020 Flotillin-dependent membrane microdomains are required for functional phagolysosomes against fungal infections. Cell Rep 32: 108017 PubMed
Dasari P, Koleci N, Shopova I, Wartenberg D, Beyersdorf N, Dietrich S, Sahagún-Ruiz A, Figge MT, Skerka C, Brakhage AA, Zipfel PF 2019 Enolase from Aspergillus fumigatus is a moonlighting and immune evasion protein that binds the human plasma complement protein factors H, FHL-1, C4BP, and Plasminogen. Front Immunol 10: 2573 PubMed
Satish S, Jimenez-Ortigosa C, Zhao Y, Lee MH, Dolgov E, Krüger T, Park S, Denning DW, Kniemeyer O, Brakhage AA, Perlin DS 2019 Stress-induced changes in the lipid microenvironment of β-(1,3)-D-glucan synthase cause clinically important echinocandin resistance in Aspergillus fumigatus. mBio 10: e00779-19 PubMed
König S, Pace S, Pein H, Heinekamp T, Kramer J, Romp E, Straßburger M, Troisi F, Proschak A, Dworschak J, Scherlach K, Rossi A, Sautebin L, Haeggström JZ, Hertweck C, Brakhage AA, Gerstmeier J, Proschak E, Werz O 2019 Gliotoxin from Aspergillus fumigatus abrogates leukotriene B4 formation through inhibition of leukotriene A4 hydrolase Cell Chem Biol 26: 1-11 PubMed
Bacher P, Hohnstein T, Beerbaum E, Röcker M, Kaufmann S, Brandt C, Röhmel J, Stervbo U, Nienen M, Babel N, Milleck J, Assenmacher M, Cornely OA, Heine G, Worm M, Creutz P, Tabeling C, Ruwwe-Glösenkamp C, Sander LE, Brunke S, Hube B, Blango M, Kniemeyer O, Brakhage AA, Schwarz C, Scheffold A 2019 Human anti-fungal Th17 immunity and pathology rely on cross-reactivity against Candida albicans. Cell 176: 1340-55 PubMed
Manfiolli AO, Siqueira FS, Dos Reis TF, Van Dijck P, Schrevens S, Hoefgen S, Föge M, Straßburger M, de Assis LJ, Heinekamp T, Rocha MC, Janevska S, Brakhage AA, Malavazi I, Goldman GH, Valiante V 2019 Mitogen-activated protein kinase cross-talk interaction modulates the production of melanins in Aspergillus fumigatus. mBio 10: e00215-00219 PubMed
Nossmann M, Boysen JM, Krüger T, König CC, Hillmann F, Munder T, Brakhage AA 2019 Yeast two-hybrid screening reveals a dual function for the histone acetyltransferase GcnE by controlling glutamine synthesis and development in Aspergillus fumigatus. Curr Genet 65: 523-38 PubMed
Shekhova E, Ivanova L, Krüger T, Stroe MC, Macheleidt J, Kniemeyer O, Brakhage AA 2019 Redox proteomic analysis reveals oxidative modifications of proteins by increased levels of intracellular reactive oxygen species during hypoxia adaptation of Aspergillus fumigatus. Proteomics 19: 1800339 PubMed
Schmidt H, Vlaic S, Krüger T, Schmidt F, Balkenhol J, Dandekar T, Guthke R, Kniemeyer O, Heinekamp T, Brakhage AA 2018 Proteomics of Aspergillus fumigatus conidia-containing phagolysosomes identifies processes governing immune evasion. Mol Cell Proteomics 17: 1084-96 PubMed
Dasari P, Shopova IA, Stroe M, Wartenberg D, Dahse HM, Beyersdorf N, Hortschansky P, Dietrich S, Cseresnyés Z, Figge MT, Westermann M, Skerka C, Brakhage AA, Zipfel PF 2018 Aspf2 from Aspergillus fumigatus recruits human immune regulators for immune evasion and cell damage. Front Immunol 9: 1635 Frontiers
Conrad T, Kniemeyer O, Henkel SG, Krüger T, Mattern DJ, Valiante V, Guthke R, Jacobsen ID, Brakhage AA, Vlaic S, Linde J 2018 Module-detection approaches for the integration of multilevel omics data highlight the comprehensive response of Aspergillus fumigatus to caspofungin. BMC Syst Biol 12: 88 PubMed
Van Dijck P, Sjollema J, Cammue BP, Lagrou K, Berman J, d'Enfert C, Andes DR, Arendrup MC, Brakhage AA, Calderone R, Cantón E, Coenye T, Cos P, Cowen LE, Edgerton M, Espinel-Ingroff A, Filler SG, Ghannoum M, Gow NAR, Haas H, Jabra-Rizk MA, Johnson EM, Lockhart SR, Lopez-Ribot JL, Maertens J, Munro CA, Nett JE, Nobile CJ, Pfaller MA, Ramage G, Sanglard D, Sanguinetti M, Spriet I, Verweij PE, Warris A, Wauters J, Yeaman MR, Zaat SAJ, Thevissen K 2018 Methodologies for in vitro and in vivo evaluation of efficacy of antifungal and antibiofilm agents and surface coatings against fungal biofilms. Microb Cell 5: 300-26 (Review) PubMed
Noßmann M, Pieper J, Hillmann F, Brakhage AA, MunderT 2018 Generation of an arginine-tRNA-adapted Saccharomyces cerevisiae strain for effective heterologous protein expression. Curr Genet 64: 589-98 PubMed
Voltersen V, Blango MG, Herrmann S, Schmidt F, Heinekamp T, Strassburger M, Krüger T, Bacher P, Lother J, Weiss E, Hünniger K, Liu H, Hortschansky P, Scheffold A, Löffler J, Krappmann S, Nietzsche S, Kurzai O, Einsele H, Kniemeyer O, Filler SG, Reichard U, Brakhage AA 2018 Proteome analysis reveals the conidial surface protein CcpA essential for virulence of the pathogenic fungus Aspergillus fumigatus. mBio 9: e01557-18 PubMed
Bergfeld A, Dasari P, Werner S, Hughes TR, Song WC, Hortschansky P, Brakhage AA, Hünig T, Zipfel PF, Beyersdorf N 2017 Direct binding of the pH-regulated protein 1 (Pra1) from Candida albicans inhibits cytokine secretion by mouse CD4+ T cells. Front Microbiol 8: 844 PubMed
Pohlers S, Martin R, Krüger T, Hellwig D, Hänel F, Kniemeyer O, Saluz HP, Van Dijck P, Ernst JF, Brakhage A, Mühlschlegel FA, Kurzai O 2017 Lipid signaling via Pkh1/2 regulates fungal CO2 sensing through the kinase Sch9. mBio 8: e02211-16 PubMed
Johns A, Scharf DH, Gsaller F, Schmidt H, Heinekamp T, Straßburger M, Oliver JD, Birch M, Beckmann N, Dobb KS, Gilsenan J, Rash B, Bignell E, Brakhage AA, Bromley MJ 2017 A nonredundant Phosphopantetheinyl transferase, PptA, is a novel antifungal target that directs secondary metabolite, Siderophore, and Lysine biosynthesis in Aspergillus fumigatus and is critical for pathogenicity. mBio 8: e01504-16 PubMed
de Vries RP, Riley R, Wiebenga A, Aguilar-Osorio G, Amillis S, Uchima CA, Anderluh G, Asadollahi M, Askin M, Barry K, Battaglia E, Bayram Ö, Benocci T, Braus-Stromeyer SA, Caldana C, Cánovas D, Cerqueira GC, Chen F, Chen W, Choi C, Clum A, Dos Santos RA, Damásio AR, Diallinas G, Emri T, Fekete E, Flipphi M, Freyberg S, Gallo A, Gournas C, Habgood R, Hainaut M, Harispe ML, Henrissat B, Hildén KS, Hope R, Hossain A, Karabika E, Karaffa L, Karányi Z, Kraševec N, Kuo A, Kusch H, LaButti K, Lagendijk EL, Lapidus A, Levasseur A, Lindquist E, Lipzen A, Logrieco AF, MacCabe A, Mäkelä MR, Malavazi I, Melin P, Meyer V, Mielnichuk N, Miskei M, Molnár ÁP, Mulé G, Ngan CY, Orejas M, Orosz E, Ouedraogo JP, Overkamp KM, Park HS, Perrone G, Piumi F, Punt PJ, Ram AF, Ramón A, Rauscher S, Record E, Riaño-Pachón DM, Robert V, Röhrig J, Ruller R, Salamov A, Salih NS, Samson RA, Sándor E, Sanguinetti M, Schütze T, Sepčić K, Shelest E, Sherlock G, Sophianopoulou V, Squina FM, Sun H, Susca A, Todd RB, Tsang A, Unkles SE, van de Wiele N, van Rossen-Uffink D, Oliveira JV, Vesth TC, Visser J, Yu JH, Zhou M, Andersen MR, Archer DB, Baker SE, Benoit I, Brakhage AA, Braus GH, Fischer R, Frisvad JC, Goldman GH, Houbraken J, Oakley B, Pócsi I, Scazzocchio C, Seiboth B, vanKuyk PA, Wortman J, Dyer PS, Grigoriev IV 2017 Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus. Genome Biol 18: 28 PubMed
Kroll K, Shekhova E, Mattern DJ, Thywissen A, Jacobsen ID, Strassburger M, Heinekamp T, Shelest E, Brakhage AA, Kniemeyer O 2016 The hypoxia-induced dehydrogenase HorA is required for coenzyme Q10 biosynthesis, azole sensitivity and virulence of Aspergillus fumigatus. Mol Microbiol 101: 92-108 PubMed
Teutschbein J, Simon S, Lother J, Springer J, Hortschansky P, Morton CO, Löffler J, Einsele H, Conneally E, Rogers TR, Guthke R, Brakhage AA, Kniemeyer O 2016 Proteomic profiling of serological responses to Aspergillus fumigatus antigens in patients with invasive aspergillosis. J Proteome Res 15: 1580-91 PubMed
Horn F, Linde J, Mattern DJ, Walther G, Guthke R, Scherlach K, Martin K, Brakhage AA, Petzke L, Valiante V 2016 Draft genome sequences of fungus Aspergillus calidoustus. Genome Announc 4: e00102-16 PubMed
Bacher P, Heinrich F, Stervbo U, Nienen M, Vahldieck M, Iwert C, Vogt K, Kollet J, Babel N, Sawitzki B, Schwarz C, Bereswill S, Heimesaat MM, Heine G, Gadermaier G, Asam C, Assenmacher M, Kniemeyer O, Brakhage AA, Ferreira F, Wallner M, Worm M, Scheffold A 2016 Regulatory T cell specificity directs tolerance versus allergy against aeroantigens in humans. Cell 167: 1067-78.e16 PubMed
Valiante V, Baldin C, Hortschansky P, Jain R, Thywißen A, Straßburger M, Heinekamp T, Brakhage AA 2016 The Aspergillus fumigatus conidial melanin production is regulated by the bifunctional bHLH DevR and MADS-box RlmA transcription factors. Mol Microbiol 102: 321-335. PubMed
Pollmächer J, Timme S, Schuster S, Brakhage AA, Zipfel PF, Figge MT 2016 Deciphering the counterplay of Aspergillus fumigatus infection and host inflammation by evolutionary games on graphs. Sci Rep 6: 27807 PubMed
Luo T, Krüger T, Knüpfer U, Kasper L, Wielsch N, Hube B, Kortgen A, Bauer M, Giamarellos-Bourboulis EJ, Dimopoulos G, Brakhage AA, Kniemeyer O 2016 Immunoproteomic analysis of antibody responses to extracellular proteins of Candida albicans revealing the importance of glycosylation for antigen recognition. J Proteome Res 15: 2394-406 PubMed
Kniemeyer O, Ebel F, Krüger T, Bacher P, Scheffold A, Luo T, Strassburger M, Brakhage AA 2016 Immunoproteomics of Aspergillus for the development of biomarkers and immunotherapies. Proteomics Clin Appl 10: 910-21 PubMed
Horn F, Habel A, Scharf DH, Dworschak J, Brakhage AA, Guthke R, Hertweck C, Linde J 2015 Draft genome sequence and gene annotation of the entomopathogenic fungus Verticillium hemipterigenum. Genome Announc 3: e01439-14 PubMed
Baldin C, Valiante V, Krüger T, Schafferer L, Haas H, Kniemeyer O, Brakhage AA 2015 Comparative proteomics of a tor inducible Aspergillus fumigatus mutant reveals involvement of the tor kinase in iron regulation. Proteomics 15: 2230-43 PubMed
Kraibooj K, Schoeler H, Svensson CM, Brakhage AA, Figge MT 2015 Automated quantification of the phagocytosis of Aspergillus fumigatus conidia by a novel image analysis algorithm. Front Microbiol 6: 549 PubMed
Altwasser R, Baldin C, Weber J, Guthke R, Kniemeyer O, Brakhage AA, Linde J, Valiante V 2015 Network modeling reveals cross talk of MAP kinases during adaptation to caspofungin stress in Aspergillus fumigatus. PLoS One 10: e0136932 PubMed
Horn F, Linde J, Mattern DJ, Walther G, Guthke R, Brakhage AA, Valiante V 2015 Draft genome sequence of the fungus Penicillium brasilianum MG11. Genome Announc 3: e00724-15 PubMed
Lin CJ, Sasse C, Valerius O, Irmer H, Heinekamp T, Straßburger M, Tran VT, Herzog B, Braus-Stromeyer SA, Braus GH 2015 Transcription factor SomA Is Required for Adhesion, Development and Virulence of the Human Pathogen Aspergillus fumigatus. PLoS Pathog 11: e1005205 PubMed
Mattern DJ, Schoeler H, Weber J, Novohradská S, Kraibooj K, Dahse HM, Hillmann F, Valiante V, Figge MT, Brakhage AA 2015 Identification of the antiphagocytic trypacidin gene cluster in the human-pathogenic fungus Aspergillus fumigatus. Appl Microbiol Biotechnol 99: 10151-61 PubMed
Krüger T, Luo T, Schmidt H, Shopova I, Kniemeyer O 2015 Challenges and strategies for proteome analysis of the interaction of human pathogenic fungi with host immune cells. Proteomes 3: 467-95 PubMed
Heinekamp T, Schmidt H, Lapp K, Pähtz V, Shopova I, Köster-Eiserfunke N, Krüger T, Kniemeyer O, Brakhage AA 2015 Interference of Aspergillus fumigatus with the immune response. Semin Immunopathol 37: 141-52 PubMed
Hillmann F, Linde J, Beckmann N, Cyrulies M, Strassburger M, Heinekamp T, Haas H, Guthke R, Kniemeyer O, Brakhage AA 2014 The novel globin protein fungoglobin is involved in low oxygen adaptation of Aspergillus fumigatus. Mol Microbiol 93: 539-53 PubMed
Horn F, Schroeckh V, Netzker T, Guthke R, Brakhage AA, Linde J 2014 Draft genome sequence of Streptomyces iranensis. Genome Announc 2: e00616-14 PubMed
Lapp K, Vödisch M, Kroll K, Strassburger M, Kniemeyer O, Heinekamp T, Brakhage AA 2014 Characterisation of the Aspergillus fumigatus detoxification systems for reactive nitrogen intermediates and impact on virulence. Front Microbiol 5: 469 PubMed
Scharf DH, Heinekamp T, Brakhage AA 2014 Human and plant fungal pathogens: the role of secondary metabolites. PLoS Pathog 10: e1003859 PLoS Pathog
Schwartze VU, Santiago AL, Jacobsen ID, Voigt K 2014 The pathogenic potential of the Lichtheimia genus revisited: Lichtheimia brasiliensis is a novel, non-pathogenic species. Mycoses 57 Suppl 3: 128-31 PubMed