Project A6

Surface proteins of Lichtheimia corymbifera as virulence determinants

Like Aspergillus fumigatus, Lichtheimia corymbifera is a ubiquitous saprophyte which can cause life-threatening infections, mainly in immunocompromised patients. While these infections (mucormycoses) are uncommon infections, they have been increasingly recognized during the last decades and are associated with high mortality rates. However, only little is known about the pathogenicity mechanisms of these fungi and therapeutic options are still limited.

The cell wall represents the outer border of fungal cells and is crucial for the stability of fungal cells under changing environmental conditions. In fungal pathogens the cell wall also plays a crucial role during the interaction with the host. The main part of the cell wall is composed of carbohydrates such as glucan, chitin and chitosan, which define the interaction with host immune cells. A variety of cell wall located proteins have also been identified in fungi, including adhesins, invasins and hydrolytic enzymes. Such surface proteins are known to contribute to the virulence of fungal pathogens and represent, due to their accessible location, interesting targets for the development of antifungal therapeutics.

In project A6, we aim to get a better understanding of the surface proteins of L. corymbifera and how they are involved in the interaction with the host. Previously, we sequenced and analyzed the genome of L. corymbifera and identified putative target proteins. Using proteomic approaches, we verified the location of several proteins on spore surfaces and identified interesting candidates such as the hydrophobic binding protein HsbA. Since the infection process also involves the hyphal stages of the fungus, stage-specific surface proteomic studies will be conducted. Based on these data we will select target proteins for in-depth molecular and immune biological studies and elucidate their role in the interaction with different host cells.

Macrophage with attached spore of Lichtheimia. Photo: Schrade, Charité Berlin.
Macrophage with attached spore of Lichtheimia. Photo: Schrade, Charité Berlin.
Principal Investigator
Dr. Kerstin Voigt
PD Dr. Kerstin Voigt

Institute of Microbiology

Department of Microbiology and Molecular Biology

Friedrich Schiller University Jena

Author Year Title Journal Links
Hassan MIA, Voigt K 2019 Pathogenicity patterns of mucormycosis: epidemiology, interaction with immune cells and virulence factors. Med Mycol 57 (Suppl 2): S245-S256 PubMed
Gryganskyi AP, Golan J, Dolatabadi S, Mondo S, Robb S, Idnurm A, Muszewska A, Steczkiewicz K, Masonjones S, Liao HL, Gajdeczka MT, Anike F, Vuek A, Anishchenko IM, Voigt K, de Hoog GS, Smith ME, Heitman J, Vilgalys R, Stajich JE 2018 Phylogenetic and phylogenomic definition of Rhizopus species. G3 (Bethesda) 8: 2007-18 PubMed
Sieber P, Voigt K, Kämmer P, Brunke S, Schuster S, Linde L 2018 Comparative study on alternative splicing in human fungal pathogens suggests its involvement during host invasion. Front Microbiol 9: 2313 Front Microbiol
Arnesen JA, Malagocka J, Gryganskyi A, Grigoriev IV, Voigt K, Stajich JE, De Fine Licht HH 2018 Early diverging insect-pathogenic fungi of the order Entomophthorales possess diverse and unique subtilisin-like serine proteases. G3 (Bethesda) 8: 3311-19 PubMed
Schulze B, Rambach G, Schwartze VU, Voigt K, Schubert K, Speth C, Jacobsen ID 2017 Ketoacidosis alone does not predispose to mucormycosis by Lichtheimia in a murine pulmonary infection model. Virulence 8: 1657-67 PubMed